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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Microbiology | 15-20% | - Identification of pathogenic microorganisms - Bacteriology and antimicrobial susceptibility - Mycology, parasitology, and virology - Specimen collection and culture techniques |
| Immunology and Serology | 5-10% | - Immune system principles and components - Infectious disease serology - Immunologic testing methodologies - Autoimmune and immunodeficiency disorders |
| Urinalysis and Other Body Fluids | 5-10% | - Semen and amniotic fluid testing - Cerebrospinal, serous, and synovial fluids - Urine formation and composition - Physical, chemical, and microscopic analysis |
| Clinical Chemistry | 20-25% | - Toxicology and liver/kidney function tests - Endocrinology and therapeutic drug monitoring - Electrolytes, acid-base balance, and blood gases - Carbohydrates, lipids, proteins, and enzymes |
| Laboratory Operations | 5-10% | - Laboratory safety and regulatory compliance - Quality control and quality assurance - Laboratory mathematics and instrumentation - Pre-analytical, analytical, and post-analytical processes |
| Blood Banking (Immunohematology) | 15-20% | - Compatibility testing - Antibody detection and identification - Blood components and storage - Transfusion reactions and adverse events - Blood group systems and antigens |
| Hematology | 20-25% | - White blood cell morphology and diseases - Red blood cell physiology and disorders - Hemostasis and coagulation testing - Routine and automated hematology procedures |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
VLDL transports endogenous lipids, whereas chylomicrons transport exogenous (dietary) lipids. Cholesterol is transported by HDL and LDL.
The function of the very low density lipoproteins (VLDL) is to transport:
- A. exogenous triglycerides
- B. cholesterol from peripheral cells to the liver
- C. endogenous triglycerides
- D. cholesterol and phospholipids to the peripheral cells
Correct Answer: C 🗳️
Normocytic- G6PD deficiency, Malaria
Microcytic- Iron deficiency, Thalassemia
Macrocytic- Chronic Liver Disease, Vitamin B12 deficiency
Hematology
Match the disease conditions with appropriate red cell size classification
1. G6PD deficiency, Malaria
2. Iron deficiency, Thalassemia
3. Chronic Liver Disease, Vitamin B12 deficiency
- A. Macrocytic
- B. Microcytic
- C. Normocytic
Correct Answer: A,B,C 🗳️
The part of the microscope that control the amount of light entering the specimen much like the iris of your eye controls light is called the iris _______________.
- A. diaphragm
- B. condenser
- C. fine adjustment
- D. objective
Correct Answer: A 🗳️
The FTA-ABS is used to confirm that a positive non-treponemal test like RPR is not the result of a biological false positive, which occur in about 1 to 10 percent of the population.
A positive RPR test and a negative FTA-ABS test is most likely the result of:
- A. Latent syphilis
- B. Secondary syphilis
- C. Primary syphilis
- D. False positive reaction
Correct Answer: D 🗳️
Charcot-Leyden crystals in stool are thought to be created from damaged eosinophil byproducts. These crystals have a strong association, although they are rare, with parasitic infections or allergic reactions.
Charcot-Leyden crystals in stool may be associated with an immune response and are thought to be the breakdown products of:
- A. monocytes
- B. neutrophils
- C. basophils
- D. lymphocytes
- E. eosinophils
Correct Answer: E 🗳️







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